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F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated
SKU: orb348093
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:7,500 - 1:30,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:3,000 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Chicken IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Rabbit F(ab')2 Anti-Chicken IgG Peroxidase Conjugated Antibody, Rabbit Fab2 Anti Chicken Peroxidase Conjugated Antibody, Rabbit Fab'2 Anti-Chicken IgG Antibody HRP Conjugation, Rabbit F(ab')2 Anti-Chicken IgY Peroxidase, Rabbit F(ab')2 Anti-Chicken IgY HRP
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F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated [orb346746]
ELISA, IHC, WB
Gallus
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ELISA results of purified F(ab')2 anti-Chicken IgG Peroxidase Conjugated Antibody tested against purified chicken IgG. Each well was coated in duplicate with 1.0 µg of chicken IgG/IgY [p/n orb2652774] (purple line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
F(ab')2 Chicken IgG (H&L) Antibody Peroxidase Conjugated (orb348093)
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