F(ab')2 Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb348094

Description

F(ab')2 Chicken IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:4,500, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Gallus
Application Notes	F(ab')2 Chicken IgG (H&L) antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Chicken IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit F(ab')2 Anti-Chicken IgG Alkaline Phosphatase Conjugated Antibody, Rabbit Fab2 Anti Chicken Alkaline Phosphatase Conjugated Antibody, Rabbit Fab'2 Anti-Chicken Antibody ALP Conjugation, Rabbit F(ab')2 Anti-Chicken IgY Alkaline Phosphatase

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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