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F(ab')2 Goat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

SKU: orb348123

Description

F(ab')2 Goat IgG (H&L) antibody (FITC)

Research Area

Cell Biology

Images & Validation

Tested ApplicationsDOT, FC, FLISA, IF
Dilution RangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
ReactivityGoat
Application Notes
F(ab')2 Anti-Goat IgG Fluorescein Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

Antibody TypeSecondary Antibody
HostDonkey
ClonalityPolyclonal
IsotypeIgG F(ab')2
ImmunogenGoat IgG whole molecule
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
ConjugationFITC

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Donkey F(ab')2 Anti-Goat IgG Antibody Fluorescein Conjugation, Donkey Fab2 Anti-Goat IgG FITC Conjugated Antibody

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Protocol Information

FC
Flow Cytometry
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IF
Immunofluorescence
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DOT
Dot Blot
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Available Sizes

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500 μl
$ 450.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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