F(ab')2 Human IgG Fc Pre-adsorbed Phycoerythrin Conjugated Antibody

SKU: orb348205

Description

F(ab')2 HUMAN IgG F(c) antibody (RPE)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, FC, IF, WB
Dilution range	FC: 1:100 - 1:250, IF: 1:100 - 1:250
Reactivity	Human
Application Notes	F(ab')2 Anti-Human IgG F(c) Phycoerythrin Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
Conjugation	RPE

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Human IgG F(c) Phycoerythrin Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Human IgG Fc Fragment Phycoerythrin Conjugated Antibody, Goat F(ab')2 Anti-Human IgG F(c) Fragment Antibody PE Conjugated

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F(ab')2 Human IgG Fc Pre-adsorbed Phycoerythrin Conjugated Antibody

Western blot of Phycoerythrin conjugated Goat F(ab')2 Anti-Human IgG F(c) Pre-Adsorbed secondary antibody. Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human IgG F(c). Other band(s): None.

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Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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DOT

Dot Blot

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