F(ab')2 Human IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed

SKU: orb348171

Description

F(ab')2 Human IgG IgA IgM (H&L) antibody (Biotin)

Images & Validation

−

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Human
Application Notes	F(ab')2 Anti-Human IgG IgA IgM (H&L) Biotin Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Read more...

Key Properties

−

Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Human IgG, IgA and IgM whole molecules
Purity	This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Mouse Serum proteins. This product is suitable for the detection of all Human immuno-globulin classes, isotypes and chain combinations.

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

Goat F(ab')2 Anti-Human IgG IgA IgM Antibody Biotin Conjugated Pre-Adsorbed, Goat Fab2 Anti-Human IgG IgA IgM Biotin Conjugated Antibody, Goat Fab2 Anti-Human IgGAM Antibody Biotin Conjugation

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols