F(ab')2 Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb348286

Description

F(ab')2 Mouse IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

−

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
Reactivity	Mouse
Application Notes	F(ab')2 Anti-Mouse IgG (Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Read more...

Key Properties

−

Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Mouse IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Conjugation	AP

Storage & Handling

−

Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.1% (w/v) Sodium Azide
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

−

Donkey F(ab')2 Anti-Mouse IgG Antibody alkaline phosphatase Conjugation, Donkey Fab2 Anti-Mouse IgG alk phos Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols