F(ab')2 Mouse IgG IgA IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb348232

Description

F(ab')2 Mouse IgG IgA IgM (H&L) antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:80,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG IgA IgM peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Mouse IgG IgA and IgM (H&L) was produced by repeated immunization with mouse IgG IgA and IgM molecule in goat.
Purity	This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-pepsin, or Human Serum Proteins. This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Thimerosal
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Mouse IgG IgA IgM Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgGAM HRP Conjugated Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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