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F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin conjugated
SKU: orb348299
Description
Images & Validation
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Item 1 of 1
| Tested Applications | DOT, FC, IF |
|---|---|
| Dilution Range | FC: 1:100 - 1:250, IF: 1:100 - 1:250 |
| Reactivity | Rabbit |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Rabbit IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
| Conjugation | RPE |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Goat F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody PE Conjugation
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