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F(ab')2 Swine IgG (H&L) Antibody Peroxidase Conjugated
SKU: orb348397
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000 |
| Reactivity | Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Swine IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG and Swine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Rabbit F(ab')2 Anti-Swine IgG Antibody Peroxidase Conjugation, Rabbit F(ab')2 Anti-Swine IgG HRP Conjugated Antibody
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ELISA Results of Rabbit F(ab')2 Anti-Swine IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 10 µg of Swine IgG (p/n orb2652739). The working dilution is 105000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS buffer, and TMB substrate (p/n orb348651).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
F(ab')2 Swine IgG (H&L) Antibody Peroxidase Conjugated (orb348397)
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