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FASLG Antibody

SKU: orb1272552

Description

FASLG Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, NeA, WB
ReactivityHuman
Application Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of FasL/Apo1L (10 ng/mL), a concentration of 0.08 - 0.1 μg/mL of this antibody is required. ELISA:To detect hFasL by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFasL. Western Blot:To detect hFasL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenProduced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL (human FAS Ligand).
TargetFASLG
PurificationAnti-hFasL specific antibody was purified by affinity chromatography employing immobilized hFasL matrix.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL

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FASLG Antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.75 ug/mL - 2.5 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a AB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

FASLG Antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.75 ug/mL - 2.5 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a AB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP48023

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FASLG Antibody (orb1272552)

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Available Sizes

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0.1 mg
¥ 10,140.00
Free Secondary Antibody (20 ul)0/0

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