FBXW7 Rabbit Polyclonal Antibody

SKU: orb580048

Description

Rabbit polyclonal antibody to FBXW7

Images & Validation

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Item 1 of 11

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the C-terminal region of Human FBXW7
Target	FBXW7
Molecular Weight	80 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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AGO, CDC4, FBW6, FBW7, hAgo, FBX30, FBXW6, SEL10, hCdc4, FBXO30, SEL-10

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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Isoforms present at 70 kDa, 66 kDa, and 59 kDa. Protein is also phosphorylated.

Sample Tissue: Human Jurkat Whole Cell, Antibody dilution: 1 ug/ml.

Sample Type: 721_B Whole Cell lysates, Antibody dilution: 1.0 ug/ml.

Sample Type: 721_B Whole Cell lysates, Antibody dilution: 1 ug/ml.

Sample Type: Human Fetal Heart, Antibody dilution: 1.0 ug/ml.

Kidney

Rabbit Anti-FBXW7 Antibody, Catalog Number: orb580048, Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue, Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes, Primary Antibody Concentration: 1:100, Other Working Concentrations: 1/600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-FBXW7 Antibody Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate. FBXW7 is supported by BioGPS gene expression data to be expressed in Jurkat.

Quick Database Links

Gene Symbol

FBXW7

UniProt

Q969H0

RefSeq (Protein):NP_001013433

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001013433

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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