Fludarabine Phosphate

SKU: orb1300713

Description

Fludarabine Phosphate is a fluorinated antimetabolite derived from vidarabine. Upon intracellular conversion to its active triphosphate form, it inhibits DNA synthesis enzymes like DNA polymerase alpha, disrupting tumor cell proliferation. It is widely used in research, including in vitro and in vivo studies, for investigating hematological cancers and chemotherapy mechanisms.

Research Area

Cell Biology, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	75607-67-9
MW	365.21
Purity	99.78%
Formula	C₁₀H₁₃FN₅O₇P
SMILES	Nc1nc(F)nc2n(cnc12)[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@@H]1O
Target	DNA/RNA Synthesis,Nucleoside Antimetabolite/Analog,Apoptosis
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.48 mM);DMSO:35 mg/mL (95.84 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);H2O:2 mg/mL (5.48 mM)

Bioactivity

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Target IC50	DNA polymerase δ:1.3 μM(Ki)\|DNA polymerase α:1.1 μM(Ki )
In Vivo	Fludarabine Phosphate effectively inhibits DNA polymerase α/δ (Ki: 1.1/1.3 μM), demonstrating potent inhibitory activity in vitro It is capable of being excised from bound DNA by DNA polymerase δ. Competitively binding with deoxyadenosine triphosphate a the A site for DNA chain elongation, Fludarabine Phosphate leads to the termination of DNA st and extension. Notably, human DNA polymerase α is more adept at incorporating Fludarabine Phosphate into DNA than polymerase δ. Read more...
In Vitro	The maximum tolerable dose of Fludarabine Phosphate is determined to be 234 mg/kg, with its LD50 (lethal dose 50%) for mice being 375 mg/kg. Notably, Fludarabine Phosphate exhibits toxicity in tumor-free mice. however, in mice carrying P388 leukemia, treatment with Fludarabine Phosphate leads to a reduction in the number of surviving cells geared towards therapy, significantly enhancin the lifespan by 110% and increasin the average survival time. Read more...
Cell Research	Cells are incubated with Fludarabine Phosphate for 5 hr and washed twice with drug-free warm medium. 800 cells are mixed with 1.3 mL of 0.25% soft agar in Dulbecco's medium supplemented with 20% fetal bovine serum (pre-warmed to 37 ℃) and incubated in a tissue culture dish for 10 days (humidified 5% CO2 , 37 ℃). At the end of the incubation period, colonies of more than 40 cells are scored under a microscope the cytotoxic effect o the drugs is expressed as a percentage of survival relative to that of untreated control cells.(Only for Reference) Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DNA polymerase α, DNA polymerase δ, DNA Synthesis, DNASynthesis, Analog, Apoptosis, Inhibitor, NSC118218, NSC-118218, NSC 118218, NSC 312887 Phosphate, inhibit, Nucleoside Antimetabolite, Nucleoside Antimetabolite/Analog, NucleosideAntimetabolite, NSC312887 Phosphate, NSC-312887 Phosphate, F-ara-A (NSC 312887) Phosphate, F-ara-AMP, fludara Phosphate, Fludarabine, Fludarabine Phosphate, RNA Synthesis, RNASynthesis

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Compound Identifiers

PubChem CID

30751 ↗

Key Properties

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No computed properties available.

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