FZD3 Rabbit Polyclonal Antibody

SKU: orb156979

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Description

Research Area

G Protein-Coupled Receptors, GPCR Signaling Pathway, Molecular Biology, Surface Molecules

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, WB
Dilution Range	WB=1:500-1000, ELISA=1:5000-10000
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human FZD3 protein (23-205/666aa)
Target	FZD3
Molecular Weight	74 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Frizzled 3; Frizzled 3 seven transmembrane spanning receptor; Frizzled family receptor 3; Frizzled homolog 3(Drosophila); Frizzled homolog 3; Frizzled-3; Frizzled3; Fz 3; Fz-3; Fz3; FZD 3; Fzd3; FZD3_HUMAN; hFz 3; hFz3.

Quality Guarantee

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Protein: brain (Rat) lysates at 30 ug, Primary: Anti-FZD3 (orb156979) at 1:300, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 74kD, Observed band size: 74kD, 25kD.

Protein: pancreas (mouse) lysates at 30 ug, Primary: Anti-FZD3 (orb156979) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 74kD, Observed band size: 74kD.

Sample: Lane 1: Human SW480 cell Lysates, Lane 2: Human U-2 OS cell Lysates, Lane 3: Human A431 cell Lysates, Lane 4: Human SH-SY5Y cell Lysates, Primary: Anti-FZD3 (orb156979) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74kDa, Observed band size: 70kDa.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated (orb156979) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated (orb156979) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated (orb156979) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

FZD3

UniProt

Q9NPG1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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