FZD4 Rabbit Polyclonal Antibody

SKU: orb330168

Description

Rabbit polyclonal antibody to FZD4

Research Area

Epigenetics & Chromatin, Molecular Biology, Signal Transduction

Images & Validation

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Item 1 of 7

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Guinea pig, Mouse, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human FZD4
Target	FZD4
Protein Sequence	Synthetic peptide located within the following region: GITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGSETVV
Molecular Weight	60 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti EVR1 antibody, anti FEVR antibody, anti FZD4S antibody, anti Fz-4 antibody, anti FzE4 antibody, anti GPCR antibody, anti MGC34390 antibody, anti CD344 antibody

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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

Sample Tissue: Human Fetal Liver, Antibody Dilution: 1.0 ug/mL.

Sample Tissue: Human Lung Tumor, Antibody Dilution: 1 ug/mL.

Sample Tissue: Human RPMI 8226 Whole Cell, Antibody Dilution: 3 ug/mL.

Positive control (+): Human lung (LU), Negative control (-): 293T (2T), Antibody concentration: 1 ug/mL.

Skin

WB Suggested Anti-FZD4 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:312500, Positive Control: Fetal Liver.

Quick Database Links

Gene Symbol

FZD4

UniProt

Q9ULV1

RefSeq (Protein):NP_036325

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_036325

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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