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Galangin

SKU: orb1303934

Description

Galangin (Norizalpinin) is a bifunctional aryl hydrocarbon receptor (AhR) agonist/antagonist that also inhibits CYP1A1 enzymatic activity. This small molecule is utilized in research to study AhR signaling pathways, xenobiotic metabolism, and related processes in both cellular and animal models.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Galangin

Key Properties

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CAS Number548-83-4
MW270.24
Purity99.93% (May vary between batches)
FormulaC15H10O5
SMILESOc1cc(O)c2c(c1)oc(-c1ccccc1)c(O)c2=O
TargetAutophagy,ERK,NF-κB,Cytochromes P450
SolubilityChloroform, Dichloromethane, Ethyl Acetate, Acetone:Soluble;10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (18.5 mM);DMSO:237 mg/mL (877 mM)

Bioactivity

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In Vitro
Through thin-layer chromatography, it has been demonstrated that Galangin impedes the catabolic decomposition of DMBA in a dose-dependent fashion and obstructs the generation of DMBA-DNA adducts, thereby averting DMBA-related suppression of cell proliferation. Additionally, Galangin robustly curtails CYP1A1 enzyme activity in a dose-responsive manner within both intact cells and DMBA-exposed cell-derived microsomes, as ascertained through ethoxyresorufin-O-deethylase activity assays. The inhibition of CYP1A1 by Galangin occurs through a noncompetitive mechanism, as evidenced by double-reciprocal plot analysis. Moreover, Galangin elevates CYP1A1 mRNA levels, suggesting potential aryl hydrocarbon receptor agonism, yet it counteracts the DMBA or TCDD-triggered upregulation of CYP1A1 mRNA and promoter-driven transcription. Furthermore, Galangin administration hampers cell proliferation while propelling autophagy at 130 μM and apoptosis at 370 μM. Specifically, in HepG2 cells, Galangin induces autophagosome accumulation, enhances microtubule-associated protein light chain 3 levels, and augments the proportion of vacuolated cells, alongside a surge in p53 expression. The Galangin-induced autophagic response is mitigated by p53 inhibition in HepG2 cells, while p53 overexpression in Hep3B cells normalizes the elevated vacuole presence.
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Cell Research
Cells (5.0×103) are seeded and treated with different concentrations of galangin for different periods of time in 96-well plates. The number of viable cells in each well is determined by adding 10 μL of 5 mg/mL MTT solution. Following the 4 hour incubation at 37°C, the cells are dissolved in a 100 μL solution containing 20% SDS and 50% dimethy formamide. The optical densities are quantified at a test wavelength of 570 nm with a reference wavelength of 630 nm using a Varioskan Flash Reader spectrophotometer.
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Storage & Handling

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StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Autophagy, 3,5,7-Trihydroxyflavone, CYPs, Cytochrome P450, ERK, Galangin, Inhibitor, NF-κB, NFκB, NFkB, NF-kB, inhibit, Norizalpinin

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID
5281616 ↗

Key Properties

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No computed properties available.

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Galangin (orb1303934)

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% Tween 80 +
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Available Sizes

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5 mg
¥ 910.00
10 mg
¥ 1,170.00
1 ml x 10 mM (in DMSO)
¥ 1,300.00
25 mg
¥ 1,560.00
50 mg
¥ 2,210.00
100 mg
¥ 3,380.00
500 mg
¥ 7,670.00
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