GANAB Rabbit Polyclonal Antibody

SKU: orb100172

This product can be custom made

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Description

GANAB Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Equine, Human, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GANAB/alpha Glucosidase II (428-540/944aa)
Target	GANAB
Molecular Weight	104 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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G2AN; GIIA; GIIalpha; GLUII; PKD3; GANAB_HUMAN; GANAB; Alpha-glucosidase 2; Glucosidase II subunit alpha; 3.2.1.207; KIAA0088;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GANAB) Polyclonal Antibody, Unconjugated (orb100172) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GANAB) Polyclonal Antibody, Unconjugated (orb100172) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Lane 3: Liver (Rat) Lysate at 40 ug, Lane 4: Lymph node (Rat) Lysate at 40 ug, Primary: Anti-GANAB (orb100172) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 104 kD, Observed band size: 110 kD.

Quick Database Links

Gene Symbol

GANAB

UniProt

Q14697

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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