GAPDH Antibody, HRP Conjugate

SKU: orb1926019

Description

Research Area

Cancer Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB - 1:40000
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Molecular Weight	36053 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody conjugated with HRP supplied in PBS with 0.05% (W/V) Proclin 300. This antibody is purified through a protein G column, followed by size-exclusion - Membrane filtration purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Anti-GAPDH Antibody, HRP Conjugate at 1:40000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody (conjugated HRP) at 1:1000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 1: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P04406

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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