GAPDH Rabbit Polyclonal Antibody

SKU: orb577464

Description

Rabbit polyclonal antibody to GAPDH

Images & Validation

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Item 1 of 8

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Goat, Guinea pig, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH
Target	GAPDH
Molecular Weight	36 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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G3PD, GAPD, HEL-S-162eP

GAPDH antibody [orb555879]

Applications:	ICC, IHC-P, IP, WB
Reactivity:	Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl

Item 1 of 7
GAPDH Rabbit Polyclonal Antibody [orb577465]
Applications:
WB
Predicted Reactivity:
Canine, Equine, Guinea pig, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl
Item 1 of 5
GAPDH Rabbit Polyclonal Antibody [orb584046]
Applications:
WB
Predicted Reactivity:
Canine, Equine, Guinea pig, Rabbit, Rat
Reactivity:
Human, Mouse, Zebrafish
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl
Item 1 of 5
GAPDH Antibody [orb1294414]
Applications:
IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 25 μl, 100 μl

Quality Guarantee

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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 84 kDa.

Sample Type: 293T, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane/lane, Gel Concentration: 0.12.

Sample Type: Hela Whole Cell lysates, Antibody Dilution: 1.0 ug/ml. GAPDH is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.

Lanes: 1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-Rabbit HRP, Secondary Antibody Dilution: 1:30000, Gene Name: GAPDH.

Rabbit Anti-GAPDH Antibody, Catalog Number: orb577464, Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue, Observed Staining: Cytoplasm, Primary Antibody Concentration: 1:600, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

Rabbit Anti-GAPDH Antibody, Catalog Number: orb577464, Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue, Observed Staining: Cytoplasm in hepatocytes, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Human brain.

WB Suggested Anti-GAPDH antibody Titration: 1 ug/ml, Sample Type: Human Raji.

Quick Database Links

Gene Symbol

GAPDH

UniProt

Q2TSD0

RefSeq (Protein):NP_002037

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002037

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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