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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 38-423 of human GATM (NP_001473.1?). |
| Molecular Weight | 48kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti AT antibody, anti AGAT antibody, anti CCDS3 antibody
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Immunofluorescence analysis of A549 cells using GATM Rabbit pAb (orb247515). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded Human liver using GATM Rabbit pAb (orb247515) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western blot analysis of various lysates using GATM Rabbit pAb (orb247515) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Chen, Hong-Ru et al. Creatine transporter deficiency impairs stress-adaptation and brain energetics homeostasis JCI Insight, 140173 (2021)
GATM Rabbit pAb (orb247515)
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