GCLC Rabbit Polyclonal Antibody

SKU: orb500689

Featured

Description

Research Area

Cell Biology, Mitochondrial, Mitochondrial Markers, Oxidative Stress

Images & Validation

−

Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Zebrafish

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GCLC (351-450/637aa)
Target	GCLC
Molecular Weight	73 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

gamma ECS; gamma GCS; gamma glutamylcysteine synthetase; gamma-ECS; gamma-glutamylcysteine synthetase; GCL; GCLC; GCS; GCS Heavy Chain antibody GLCL; GLCLC; GLCLR; Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase(gamma glutamylcysteine synthetase) regulatory(30.8kD); Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase modifier subunit; Glutamate cysteine ligase regulatory protein; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (orb500689) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Kidney (Rat) Lysate at 40 ug, Primary: Anti-GCLC (orb500689) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73 kD, Observed band size: 73 kD.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-GCLC (orb500689) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73 kDa, Observed band size: 73 kDa.

Quick Database Links

Gene Symbol

GCLC

UniProt

P48506

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols