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GDF15 Antibody

SKU: orb1537796

Description

GDF15 Antibody

Images & Validation

Tested ApplicationsELISA, FC, IHC, IHC-P, WB
Dilution rangeFlo (10 µg/ml), IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:2000), WB (0.1 - 0.3 µg/ml)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-QKTDTGVSLQTYDD, from the C-Terminus of protein sequence according to NP_004855.2.
TargetGDF15
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated gene 1 protein, PLAB, NSAID-activated gene 1 protein, Placental TGF-beta, PTGFB, GDF-15, PTGF-beta

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Datasheet
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GDF15 Antibody (orb1537796)

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Available Sizes

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50 μg
¥ 8,710.00
DispatchUsually dispatched within 2-3 weeks
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