General 1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit

SKU: orb781701

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1-Desamino 8D Arginine Vasopressin(DDAVP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1-Desamino 8D Arginine Vasopressin(DDAVP).

Research Area

Cardiovascular Research, Metabolism Research

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DDAVP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DDAVP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DDAVP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	All
Sample Types	Serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	156.25-10000 pg/mL
Sensitivity	38.4 pg/mL
Concentration	10000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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d-DAVP ELISA Kit, Stimate ELISA Kit, Minirin ELISA Kit, Desmopressin ELISA Kit

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General 1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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