General Estradiol (E2) ELISA Kit

SKU: orb1946763

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Description

Research Area

Pharmacology & Drug Discovery

Images & Validation

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Item 1 of 1

Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	All
Sample Types	Serum, Plasma, Other biological fluids,Sample volume: 50μL
Assay Type	Competitive
Assay Time	1.5H
Range	12.5-800pg/mL
Sensitivity	7.5 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol

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