GFAP Antibody / Glial Fibrillary Acidic Protein

SKU: orb1825047

Description

GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the GFAP antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	GFAP/8616R
Immunogen	A recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the GFAP antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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GFAP Antibody / Glial Fibrillary Acidic Protein

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/8616R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/8616R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P14136

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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