Recombinant GFAP Antibody / Rabbit Monoclonal

SKU: orb606415

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Research Area

Neuroscience

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	ASTRO/1974R
Immunogen	GFAP isolated from pig spinal cord was used as the immunogen for this recombinant GFAP antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

UniProt

P14136

UniProt Details

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No UniProt data available

Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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Available Sizes

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20 μg

$ 370.00

100 μg

$ 750.00

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Recombinant GFAP Antibody / Rabbit Monoclonal

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

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