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GFAP Antibody
SKU: orb749357
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FACS, IHC-P, WB |
|---|---|
| Dilution range | Flow cytometry: 1-2ug/million,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | GA-5 |
| Immunogen | GFAP isolated from pig spinal cord was used as the immunogen. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1X PBS, pH 7.4 |
| Disclaimer | For research use only |
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IHC staining of FFPE human cerebellum with GFAP antibody (clone GA-5).
Western blot testing of human brain lysate GFAP antibody (clone GA-5). Predicted molecular weight ~50 kDa.
Western blot testing of human brain lysate GFAP antibody (clone GA-5). Predicted molecular weight ~50 kDa.
Flow cytometry testing of permeabilized human T98G cells with GFAP antibody (clone GA-5); Red = isotype control, Blue = GFAP antibody.
SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GA-5) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
GFAP Antibody (orb749357)
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