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GFAP Antibody

SKU: orb749517

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsIHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
ReactivityHuman, Rat
Application Notes
Optimal dilution of the GFAP antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.ASTRO/789
ImmunogenFull length recombinant protein was used as the immunogen for the GFAP antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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GFAP Antibody

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (ASTRO/789).

GFAP Antibody

IHC: Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP antibody (ASTRO/789).

GFAP Antibody

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with GFAP antibody (ASTRO/789).

GFAP Antibody

Western blot testing of human brain lysate with GFAP antibody (clone ASTRO/789). Predicted molecular weight ~50 kDa.

GFAP Antibody

SDS-PAGE Analysis of Purified, BSA-Free GFAP Antibody (clone ASTRO/789). Confirmation of Integrity and Purity of the Antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol

GFAP Antibody (orb749517)

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Available Sizes

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20 μg
¥ 4,810.00
100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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