GFAP Antibody Cocktail

SKU: orb749518

Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Research Area

Epigenetics

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the GFAP antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	GA-5 + ASTRO/789
Immunogen	GFAP isolated from pig spinal cord (GA-5) and recombinant protein (ASTRO/789) were used as the immunogen for the GFAP antibody cocktail.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP antibody cocktail (GA-5 + ASTRO/789).

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with GFAP antibody cocktail (GA-5 + ASTRO/789).

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody cocktail (GA-5 + ASTRO/789).

Western blot testing of human brain lysate with GFAP antibody cocktail (clones GA-5 + ASTRO/789). Predicted molecular weight ~50 kDa.

Quick Database Links

UniProt

P14136

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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