Glial Fibrillary Acidic Protein Antibody

SKU: orb750205

Description

Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Research Area

Epigenetics

Images & Validation

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Tested Applications	FACS, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
Reactivity	Human
Application Notes	The optimal dilution of the Glial Fibrillary Acidic Protein antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	SPM248
Immunogen	GFAP isolated from pig spinal cord was used as the immunogen for this Glial Fibrillary Acidic Protein antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (clone SPM248).

Western blot testing of human T98G cell lysate with Glial Fibrillary Acidic Protein antibody (clone SPM248). Expected molecular weight 50~55 kDa.

Flow cytometry testing of permeabilized human T98G cells with Glial Fibrillary Acidic Protein antibody (clone SPM248); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.

SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone SPM248) as confirmation of integrity and purity.

Quick Database Links

UniProt

P14136

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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