GFAP Antibody (N-term)

SKU: orb1931853

Description

Research Area

Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB - 1:1000
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	49880 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

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Western blot analysis of GFAP (arrow) using GFAP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2).

All lanes: Anti-GFAP Antibody (M1) at 1: 8000 dilution. Lane 1: human brain lysate. Lane 2: human cerebellum lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P14136

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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