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GFAP Antibody
SKU: orb1259841
Featured
Description
Research Area
Cell Biology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1). |
| Target | GFAP |
| Molecular Weight | Observed: 50kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Glial fibrillary acidic protein, GFAP, GFAP
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Western blot analysis of extracts of various cell lines, using GFAP antibody (orb1259841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (orb1259841) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
GFAP
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
GFAP Antibody (orb1259841)
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