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GFAP Antibody
SKU: orb1338576
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB 1:2000, IHC 1:50, IF 1:100 |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | OTI2C4 |
| Immunogen | Full length human recombinant protein of human GFAP (NP_002046) produced in HEK293T cell. |
| Target | GFAP |
| Molecular Weight | 49.9 kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
Anti-GFAP mouse monoclonal antibody (TA500335) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
GFAP Antibody (orb1338576)
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