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GFAP Antibody
SKU: orb150514
Featured
Description
Research Area
Disease Biomarkers, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution range | WB (1:1000) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | N206A/8 (Formerly sold as S206A-8) |
| Immunogen | Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse |
| Target | GFAP |
| Molecular Weight | 50kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−GFAP, Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, GFAPL, DKFZp459C0729, MGC139638, FLJ45472, AI836096
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP Monoclonal Antibody, Clone N206A/8. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GFAP Antibody (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone N206A/8. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:250.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001124491.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
GFAP Antibody (orb150514)
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