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GFAP Antibody
SKU: orb627313
Featured
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:5000-1:50000; IP: 1:500-1:5000; IHC: 1:500-1:10000; IF: 1:50-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | glial fibrillary acidic protein |
| Target | GFAP |
| Molecular Weight | 45-50 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
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IP Result of anti-GFAP (IP: orb627313, 5 ug; Detection: orb627313 1:1000) with mouse brain tissue lysate 2640 ug.
U-251 cellswere subjected to SDS PAGE followed by western blot with orb627313 (GFAP antibody) at dilution of 1:10000.
Immunohistochemistry of paraffin-embedded human brain tissue slide using orb627313 (GFAP Antibody) at dilution of 1:500 Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
Quick Database Links
Gene Symbol
GFAP
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
GFAP Antibody (orb627313)
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