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GIT1-Y554 Antibody (C-term)
SKU: orb1929280
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB - 1:1000, FC - 1:10-50 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 84341 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
GIT1-Y554 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).
GIT1-Y554 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).
GIT1-Y554 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilution. Lane 1: U-2OS whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
GIT1-Y554 Antibody (C-term) (orb1929280)
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