GLI2 Rabbit Polyclonal Antibody

SKU: orb329627

Description

Rabbit polyclonal antibody to GLI2

Research Area

Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 6

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Human

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
Target	GLI2
Protein Sequence	Synthetic peptide located within the following region: RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE
Molecular Weight	133kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti HPE9 antibody, anti THP1 antibody, anti THP2 antibody

Quality Guarantee

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Sample Tissue: Human RPMI 8226 Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Type: HepG2 Whole Cell lysates, Antibody Dilution: 1.0 ug/mL.

Sample Type: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5 ug/mL, Peptide Concentration: 4.0 ug/mL, Lysate Quantity: 25 ug/lane, Gel Concentration: 6%-18%.

Rabbit Anti-GLI2 Antibody, Paraffin Embedded Tissue: Human Heart, Cellular Data: Myocardial cells, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Rabbit Anti-GLI2 Antibody, Paraffin Embedded Tissue: Human Intestine, Cellular Data: Epithelial cells of intestinal gland, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Sample Type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop, Primary Antibody Dilution: 1:50, Secondary Antibody: Goat anti-rabbit-cy3, Secondary Antibody Dilution: 1:1500, Color/Signal Descriptions: GLI2: Green Neural crest cells: Red, Gene Name: GLI2.

Quick Database Links

Gene Symbol

GLI2

UniProt

P10070

RefSeq (Protein):NP_084655

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_084655

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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