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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This GLS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 498-524 amino acids from the C-terminal region of human GLS2. Antigen Region: 498-524 aa. |
| Target | GLS2 |
| Molecular Weight | 66323 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GLS2; GA; Glutaminase liver isoform, mitochondrial; L-glutaminase; L-glutamine amidohydrolase
Quality Guarantee
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Immunohistochemical analysis of paraffin-embedded H.brain section using GLS2 Antibody (C-term E513). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from human brain, mouse brain, rat brain tissue lysate (from left to right), using GLS2 Antibody (C-term E513). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
GLS2 antibody (C-term E513) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS2 antibody (C-term E513) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_037399.2, NP_001267727.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
GLS2 Antibody (C-term E513) (orb1788150)
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