Recombinant GYPA Antibody / Glycophorin A

SKU: orb2640661

Description

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]

Images & Validation

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Tested Applications	IHC-P
Dilution range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the recombinant GYPA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rGYPA/280
Immunogen	Recombinant human Glycophorin A protein was used as the immunogen for the recombinant GYPA antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant GYPA Antibody / Glycophorin A

IHC testing of FFPE human angiosarcoma with recombinant GYPA antibody (clone rGYPA/280). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant GYPA antibody (clone rGYPA/280) as confirmation of integrity and purity.

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UniProt

P02724

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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