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58KGolgi protein(Internal)/FTCD Antibody
SKU: orb18891
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml |
| Reactivity | Human, Mouse, Porcine |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | 58KGolgi protein(Internal)/FTCD |
| Molecular Weight | 58.9; 58.9; 61.3 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti FTCD antibody, anti LCHC1 antibody, anti formiminotransferase cyclodeaminase antibody, anti formimidoyltransferase cyclodeaminase antibody, anti LC1 autoantigen antibody
Quality Guarantee
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0.75 µg/ml staining of Human (A) and Mouse (B) and (0.5 ug/ml) of Rat Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
6 µg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
58KGolgi protein(Internal)/FTCD Antibody (orb18891)
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