ACO1/Aconitase 1 Antibody

SKU: orb20022

Description

Goat polyclonal to ACO1

Research Area

Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:8000, WB: 0.1-0.3 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 100kDa band observed in Human Liver lysates (calculated MW of 98.4kDa according to NP_002188.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	ACO1 / Aconitase 1
Protein Sequence	QVDFNRRADSLQKNQ
Molecular Weight	98.4
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti ACO1 antibody, anti aconitase 1, soluble antibody, anti ACONS antibody, anti IREB1 antibody, anti IREBP antibody, anti IREBP1 antibody, anti IRP1 antibody, anti OTTHUMP00000021176 antibody, anti OTTHUMP00000021177 antibody, anti OTTHUMP00000045233 antibody, anti aconitase 1 antibody, anti aconitate hydratase antibody, anti citrate hydro-lyase antibody, anti ferritin repressor protein antibody, anti iron regulatory protein 1 antibody, anti iron-responsive element binding protein 1 antibody

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orb20022 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ACO1 / Aconitase 1

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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