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AKT3 Antibody
SKU: orb18912
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | AKT3 |
| Protein Sequence | CSPTSQIDNIGEEEM |
| Molecular Weight | 55.8; 54.0 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti AKT3 antibody, anti STK-2 antibody, anti RAC-gamma antibody, anti RAC-PK-gamma antibody, anti DKFZP434N0250 antibody, anti protein kinase B gamma antibody, anti serine threonine protein kinase, Akt-3 antibody, anti RAC-gamma serine/threonine protein kinase antibody
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1 µg/ml staining of HepG2 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
1 µg/ml staining of Human Thyroid (A), (0.5 ug/ml) Mouse Brain (B) and (1 µg/ml) Rat Brain (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
5 µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
5 µg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed THP-1 immobilized on Shi-fix™ plus cover-slips. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed A431. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
AKT3 Antibody (orb18912)
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