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Description
Research Area
Neuroscience
Images & Validation
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Item 1 of 3
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx. 27kDa band observed in lysates of cell line A431, and approx. 26kDa in lysates of cell line A549 and in Human Heart lysates (calculated MW of 31.7kDa according to NP_658985.2). Recommended concentration: 3µg/ml. Primary incubation 1 hour at room temperature. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | APOA1BP |
| Protein Sequence | PPYPDTECVYRLQ |
| Molecular Weight | 31.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti apolipoprotein A-I binding protein antibody, anti AIBP antibody, anti MGC119143 antibody, anti MGC119144 antibody, anti MGC119145 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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