Goat anti-BAG3 / BIS/ CAIR1 Antibody

SKU: orb18594

Description

Goat polyclonal antibody to BAG3

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 85kDa band observed in Human Skeletal Muscle lysates (calculated MW of 61.6kDa according to NP_004272.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Iwasaki et al, Cancer Res. 2007 Nov 1;67(21):10252-9. PMID: 17974966). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	BAG3 / BIS/ CAIR1
Molecular Weight	61.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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Anti-BAG 3 antibody, anti-BAG family molecular chaperone regulator 3 antibody, anti-BAG-3 antibody, anti-Bag3 antibody, anti-BAG3_HUMAN antibody, anti-Docking protein CAIR 1 antibody, anti-Docking protein CAIR-1. antibody

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Western blot analysis of Human Skeletal Muscle lysate using BAG3 antibody

Immunohistochemical staining of Human Heart using BAG3 antibody

Immunohistochemical staining of Human Skeletal Muscle using BAG3 antibody

Immunohistochemical staining of Human Placenta using BAG3 antibody

Quick Database Links

Gene Symbol

BAG3 / BIS/ CAIR1

NCBI Gene ID

9531

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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