BIF-1/SH3GLB1 Antibody

SKU: orb18342

Description

Goat polyclonal antibody to SH3GLB1

Images & Validation

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Tested Applications	ELISA, FC, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.3-1.0 μg/ml, IHC-P: 2.5 μg/ml
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 μg/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	BIF-1 / SH3GLB1
Molecular Weight	40.8; 44.3; 43.3; 29.3
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti SH3GLB1 antibody, anti Bif-1 antibody, anti SH3-domain GRB2-like endophilin B1 antibody, anti CGI-61 antibody, anti KIAA0491 antibody, anti endophilin B1 antibody, anti Bax-interacting factor 1 antibody, anti SH3-containing protein SH3GLB1 antibody, anti SH3-domain, GRB2-like, endophilin B1 antibody, anti SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1 antibody, anti dJ612B15.2 antibody

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Western blot analysis of Jurkat lysate using SH3GLB1 antibody

Immunohistochemical staining of Human Colon using SH3GLB1 antibody

Quick Database Links

Gene Symbol

BIF-1 / SH3GLB1

NCBI Gene ID

51100

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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