BLNK/SLP-65 Antibody

SKU: orb18354

Description

Goat polyclonal antibody to BLNK

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, WB
Dilution range	ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to 037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50kDa band is observed. Recommended concentration: 1-3 μg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	BLNK / SLP-65
Molecular Weight	50.5; 48.2; 44.5; 42.2; 33.0
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti BLNK antibody, anti SLP-65 antibody, anti B-cell linker antibody, anti BASH antibody, anti BLNK-s antibody, anti Ly57 antibody, anti MGC111051 antibody, anti SLP65 antibody, anti B cell linker protein antibody, anti B-cell adapter containing a SH2 domain protein antibody, anti B-cell adapter containing a Src homology 2 domain protein antibody, anti OTTHUMP00000020167 antibody, anti Src homology 2 domain-containing leukocyte protein of 65 kDa antibody, anti BLNK-S antibody, anti LY57 antibody, anti OTTHUMP00000020168 antibody

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Western blot analysis of Daudi lysate using BLNK antibody

Western blot analysis of HEK293 cells using BLNK antibody

Immunofluorescence analysis of HepG2 cells using BLNK antibody

Flow cytometric analysis of Daudi cells using BLNK antibody

Quick Database Links

Gene Symbol

BLNK / SLP-65

NCBI Gene ID

29760

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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