Cathepsin B Antibody

SKU: orb536206

Description

Goat polyclonal antibody to CTSB

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC
Dilution range	IF/ICC: 10 μg/ml, P-ELISA: 1:64000, IHC-P: 5 μg/ml, FACS: 10ug/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse, Rat
Application Notes	IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and USO2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Cathepsin B
Molecular Weight	37.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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CTSB, cathepsin B , HGNC:2527, APPS, CPSB, APP secretase, amyloid precursor protein secretase, cathepsin B1 cysteine protease, preprocathepsin B

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Immunofluorescence analysis of U2OS cells using CTSB antibody

Immunofluorescence analysis of HeLa cells using CTSB antibody

Flow cytometric analysis of HepG2 cells using CTSB antibody

Immunohistochemical staining of Human Small Intestine using CTSB antibody

Quick Database Links

Gene Symbol

Cathepsin B

NCBI Gene ID

1508

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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