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Caveolin 1 Antibody

SKU: orb19043

Description

Goat polyclonal antibody to Caveolin 1

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution rangeELISA: 1:8000, WB: 0.001-0.01 μg/ml, IHC-P: 2.5μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml.
ReactivityHuman
Predicted ReactivityCanine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 21kDa and 25kDa observed in Human Heart, Lung and Spleen lysates. These bands correspond to earlier findings in literature with different antibodies (Wiechen et al Am J Pathol. 2001 Mar;158(3):833-9, PMID: 11238032 ). In transfected HEK293 transiently expressing Caveolin 1 a band of approx. 23kDa is observed. This band is not observed in the non-transfected HEK293. This protein has a calculated MW of 20.5kDa according to NP_001744.2. Recommended concentration: 0.3-1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetCaveolin 1
Molecular Weight20.5; 17.0
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti BSCL3 antibody; anti CAV 1 antibody; anti CAV antibody; anti CAV1 antibody; anti Caveolin-1 antibody; anti Caveolin1 antibody; anti cell growth-inhibiting protein 32 antibody; anti CGL3 antibody; anti LCCNS antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
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IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Caveolin 1 Antibody (orb19043)

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Available Sizes

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100 μg
¥ 6,890.00
DispatchUsually dispatched within 5-10 working days
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