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CCKBR Antibody

SKU: orb19186

Description

Goat polyclonal antibody to Gastrin Receptor

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 6 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows strong staining in islets of Langerhans and weak staining in acinar cells. Recommended concentration, 3ug/ml.WB: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J, Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetCCKBR
Molecular Weight48.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

Anti-G14 antibody, anti-G17 antibody, anti-G34 antibody, anti-G52 antibody, anti-G6 antibody, anti-GAS antibody, anti-Gast antibody, anti-GAST_HUMAN antibody, anti-Gastrin antibody, anti-Gastrin component I antibody, anti-Gastrin component II antibody, anti-Gastrin component III antibody, anti-Gastrin precursor antibody, anti-Gastrin-14 antibody, anti-GASR_HUMAN antibody,anti-GASR antibody, anti-Gastrin receptor antibody

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Western blot analysis Mouse Pancreas lysate using CCKBR antibody

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Immunohistochemical staining of Human Pancreas using CCKBR antibody

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Western blot analysis of Mouse Pancreas(lane 1) and Rat Stomach(lane 2) lysate using CCKBR antibody

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Immunohistochemical staining of Human Pancreas using CCKBR antibody

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Immunohistochemical staining of Pancreas using CCKBR antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CCKBR Antibody (orb19186)

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Available Sizes

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100 μg
¥ 6,500.00
DispatchUsually dispatched within 5-10 working days
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