Cdc42-binding kinase alpha Antibody

SKU: orb18762

Description

Goat polyclonal antibody to CDC42BPA

Images & Validation

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Tested Applications	ELISA, IHC
Dilution range	ELISA: 1:16000, IHC-P: 3.75 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Cdc42-binding kinase alpha
Molecular Weight	186, 196
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti CDC42BPA antibody, anti MRCK antibody, anti MRCKA antibody, anti PK428 antibody, anti FLJ23347 antibody, anti KIAA0451 antibody, anti CDC42 binding protein kinase alpha (DMPK-like) antibody, anti ser-thr protein kinase PK428 antibody, anti myotonic dystrophy kinase-related CDC42-binding protein kinase alpha antibody, anti Ser-Thr protein kinase related to the myotonic dystrophy protein kinase antibody, anti DKFZp686L1738 antibody, anti CDC42 binding protein kinase beta antibody

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Immunohistochemical staining of Human Cerebellum using CDC42BPA antibody

Quick Database Links

Gene Symbol

Cdc42-binding kinase alpha

NCBI Gene ID

8476

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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