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CTGF Antibody
SKU: orb421982
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution range | ELISA: 1:64000, IHC-P: 10ug/ml |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Porcine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | CTGF |
| Molecular Weight | 38.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−CTGF; connective tissue growth factor; CCN2; HCS24; IGFBP8; MGC102839; NOV2; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; OTTHUMP00000017213; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
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Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
6 μg/ml staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CTGF Antibody (orb421982)
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